RT Journal Article
SR Electronic
T1 Multiple breakpoint method for measuring effect of antibiotics on endocarditis strains of streptococci.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 774
OP 776
DO 10.1136/jcp.43.9.774
VO 43
IS 9
A1 J Marks
A1 A Paull
YR 1990
UL http://jcp.bmj.com/content/43/9/774.abstract
AB The activity of penicillin alone and combined with aminoglycoside on endocarditis strains of streptococci was examined. Good assay reproducibility was obtained by the use of logarithmic phase cultures standardised by opacity, careful inoculation of well-plates, removal of antibiotic by membrane transfer and incubating survival counts in hydrogen plus carbon dioxide. The use of 10-fold intervals for penicillin concentration simplified assay design without loss of efficiency.