RT Journal Article
SR Electronic
T1 Simple technique for detecting RNA viruses by PCR in single sections of wax embedded tissue.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 276
OP 277
DO 10.1136/jcp.46.3.276
VO 46
IS 3
A1 C J Woodall
A1 N J Watt
A1 G B Clements
YR 1993
UL http://jcp.bmj.com/content/46/3/276.abstract
AB The detection of specific RNA species in wax-embedded tissue sections using the polymerase chain reaction (PCR) means that gene expression can be studied and RNA viruses detected in stored histological tissue samples. This technique potentially allows the distribution of gene expression and viral replication to be studied in finely subdivided tissues. A technique is presented that has been used successfully to detect short RNA target sequences (130-420 bases) from proto-oncogene Abelson, human enteroviruses, and the sheep retrovirus Maedi-Visna virus using RNA PCR in single wax sections (20-30 microns). Various tissues were used which had not been deliberately prepared for this purpose. In a simple procedure hot xylene dewaxing is followed by acid phenol extraction of RNA and RNA PCR.