RT Journal Article
SR Electronic
T1 PCR amplification of DNA from stained cytological smears.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 378
OP 379
DO 10.1136/jcp.46.4.378
VO 46
IS 4
A1 Gall, K
A1 Pavicić, D
A1 Pavelić, J
A1 Audy-Jurković, S
A1 Pavelić, K
YR 1993
UL http://jcp.bmj.com/content/46/4/378.abstract
AB DNA from archival Papanicolaou stained smears was successfully amplified using the polymerase chain reaction (PCR) to see if it could be used for retrospective genome studies such as detection of the presence of human papilloma virus (HPV) and changes in p53 gene expression. DNA was isolated and purified by treatment with proteinase K, phenol/chloroform, and isoamyl alcohol. Segments of the human beta actin and TGF beta 1 gene were amplified by PCR. Of all stains used in the preparation of Papanicolaou smears, only eosin was detectable as a greenish band in ethidium bromide treated DNA gels under ultraviolet illumination.