TY - JOUR T1 - Detection of the C protein gene among group B streptococci using PCR. JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 633 LP - 636 DO - 10.1136/jcp.46.7.633 VL - 46 IS - 7 AU - J A Mawn AU - A J Simpson AU - S R Heard Y1 - 1993/07/01 UR - http://jcp.bmj.com/content/46/7/633.abstract N2 - AIM--To develop a polymerase chain reaction (PCR) for the specific detection of the C protein gene in strains of group B Streptococcus. METHODS--A single primer pair derived from the nucleotide sequence of the IgA binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA fragment from the C protein gene. After 35 cycles of amplification this product could be detected by agarose gel electrophoresis. Southern blot hybridisation confirmed that this product was the C protein gene. RESULTS--PCR detected the C protein gene in 75 (63%) of 119 strains of group B streptococci analysed. The product was not detected in other Gram positive organisms, showing that this PCR assay was highly specific. The sensitivity of the assay was satisfactory to a dilution of 1 in 10,000 of extracted DNA. CONCLUSIONS--The C protein of group B streptococci is associated with neonatal sepsis. The specific detection of the C protein gene by PCR may help identify which strains are likely to be associated with infection by the organism. ER -