@article {Marques1089, author = {M E Marques and K I Coelho and M N Sotto and C E Bacchi}, title = {Comparison between histochemical and immunohistochemical methods for diagnosis of sporotrichosis.}, volume = {45}, number = {12}, pages = {1089--1093}, year = {1992}, doi = {10.1136/jcp.45.12.1089}, publisher = {BMJ Publishing Group}, abstract = {AIMS: To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. METHODS: Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori{\textquoteright}s methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxidase) (ABC) technique associated with a newly produced rabbit polyclonal antibody anti-Sporothrix schenckii. RESULTS: A total of 29 (83\%) cases were positive by the ABC method used in association with anti-Sporothrix schenckii rabbit polyclonal antibodies. Histochemical methods, using silver staining, periodic acid Schiff, and conventional haematoxylin and eosin detected 37\%, 23\%, and 23\% of forms of S schenckii, respectively. The ABC technique was significantly more reliable than periodic acid Schiff and silver staining techniques. CONCLUSIONS: It is concluded that immunostaining is an easy and rapid method which can efficiently increase the accuracy of the diagnosis of sporotrichosis in human tissue.}, issn = {0021-9746}, URL = {https://jcp.bmj.com/content/45/12/1089}, eprint = {https://jcp.bmj.com/content/45/12/1089.full.pdf}, journal = {Journal of Clinical Pathology} }