RT Journal Article
SR Electronic
T1 Pulsed field gel electrophoresis on frozen tumour tissue sections.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 722
OP 723
DO 10.1136/jcp.45.8.722
VO 45
IS 8
A1 J Boultwood
A1 L Kaklamanis
A1 K C Gatter
A1 J S Wainscoat
YR 1992
UL http://jcp.bmj.com/content/45/8/722.abstract
AB The application of pulsed field gel electrophoresis (PFGE) to the molecular genetic analysis of solid tumours has been restricted by the requirement for whole single cells as a DNA source. A simple technique which allows for the direct analysis of histologically characterised solid tumour material by pulsed field gel electrophoresis was developed. Single frozen tissue sections obtained from colonic carcinoma specimens were embedded without further manipulation in molten, low melting temperature agarose. The tumour DNA contained within the agarose plug was subjected to restriction enzyme digestion and PFGE. Sufficient high molecular weight DNA is yielded by this method to obtain a hybridisation signal with a single copy probe. Histological examination of adjacent tissue sections may also be carried out, permitting correlation between molecular analysis and tumour histology.