RT Journal Article
SR Electronic
T1 PCR analysis of the upstream regulatory region of human papillomavirus genomes in cervical intraepithelial neoplasia and cervical carcinoma.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1021
OP 1023
DO 10.1136/jcp.46.11.1021
VO 46
IS 11
A1 Y K Donaldson
A1 M J Arends
A1 E Duvall
A1 C C Bird
YR 1993
UL http://jcp.bmj.com/content/46/11/1021.abstract
AB AIMS--To test whether human papillomavirus (HPV) variants with large scale sequence alterations to the upstream regulatory region are present in cervical intraepithelial neoplasias (CIN) and cervical carcinomas. METHODS--New PCR based assays were designed specifically to detect large scale insertion/deletion alterations in the upstream regulatory region of HPV 16 and 18. The assays were applied to 24 cases of CIN and 34 cases of cervical carcinoma previously shown to contain these two high risk HPV types. RESULTS--No large scale sequence alterations were found in any of the HPV containing CIN or carcinomas. CONCLUSIONS--These negative findings suggest that major upstream regulatory region variants of HPV 16 and 18 do not contribute to most cervical neoplasms.