PT  - JOURNAL ARTICLE
AU  - Gururangan, S
AU  - Dorman, A
AU  - Ball, R
AU  - Curran, B
AU  - Leader, M
AU  - Breatnach, F
AU  - O'Meara, A
TI  - DNA quantitation of Wilms' tumour (nephroblastoma) using flow cytometry and image analysis.
AID  - 10.1136/jcp.45.6.498
DP  - 1992 Jun 01
TA  - Journal of Clinical Pathology
PG  - 498--501
VI  - 45
IP  - 6
4099  - http://jcp.bmj.com/content/45/6/498.short
4100  - http://jcp.bmj.com/content/45/6/498.full
SO  - J Clin Pathol1992 Jun 01; 45
AB  - AIMS: To compare flow cytometry (FCM) with image analysis (IA) in the DNA quantitation of Wilms' tumour (WT) and to correlate data so obtained with recognised clinical and pathological prognostic parameters. METHODS: Thirty six patients with histologically proved WT diagnosed between 1980-89 were investigated. Fifteen patients had stage I disease, 10 stage II, six stage III, two stage IV and three stage V. Suspension of nuclei obtained by pepsin digestion of paraffin wax embedded tumour tissue was analysed using a FAC-Scan flow cytometer, and a CAS-100 image analyser. RESULTS: Tumours were concordant in most instances, however, IA identified aneuploidy in two tumour samples which were diploid by FCM. Aneuploidy was detected in 5/33 tumours with favourable histology and 3/3 with unfavourable histology. Three of 28 patients with Stage I, II and V disease and 5/8 patients with stage III and IV had aneuploid tumours. All patients with unfavourable histology died of disease. In the group with favourable histology, 4/5 patients with aneuploid tumours developed recurrent disease compared with 1/27 diploid tumours (p less than 0.0001). CONCLUSIONS: Ploidy may be a useful additional prognostic indicator in Wilms' tumour with favourable histology. Larger scale studies are needed to confirm the relation of ploidy to survival in early stage WT.