RT Journal Article
SR Electronic
T1 Evaluation of modified passive haemagglutination assay for Vi antibody estimation in Salmonella typhi infections.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 740
OP 741
DO 10.1136/jcp.45.8.740
VO 45
IS 8
A1 G Kang
A1 G Sridharan
A1 M V Jesudason
A1 T J John
YR 1992
UL http://jcp.bmj.com/content/45/8/740.abstract
AB A simple passive haemmagglutination assay (PHA) was developed to detect Vi antibodies, to improve the diagnosis of typhoid fever by small laboratories. The Vi capsular antigen of Salmonella typhi was extracted by alternate alcohol and acetone precipitation. Formalin fixed, sheep red blood cells treated with chromium chloride were sensitised with this Vi antigen and antibodies detected and measured by PHA. The test had a sensitivity of 83.3% among 30 cases of typhoid fever confirmed by culture. The specificity of the test was 94%, making it suitable for use in laboratories without facilities for IFAT or ELISA.