RT Journal Article
SR Electronic
T1 Serological reactivity against cyst and tachyzoite antigens of Toxoplasma gondii determined by FAST-ELISA.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 908
OP 911
DO 10.1136/jcp.48.10.908
VO 48
IS 10
A1 Y W Zhang
A1 A Fraser
A1 A H Balfour
A1 T G Wreghitt
A1 J J Gray
A1 J E Smith
YR 1995
UL http://jcp.bmj.com/content/48/10/908.abstract
AB AIMS--To obtain quantitative data on the human serological response to Toxoplasma gondii tachyzoite and bradyzoite antigens. METHODS--Serum samples from 30 patients who had positive antibody titres against T gondii and from 14 who were seronegative, together with sequential serum samples from four infected individuals, were screened by FAST-ELISA. RESULTS--Serum samples from the 30 seropositive patients showed high IgG and IgM titres against the T gondii tachyzoite antigen but very low responses to cyst antigen. This result was borne out in sequential serum samples from patients with toxoplasmosis. CONCLUSION--Antibody recognition of the cystic stage of T gondii is low, implying that either this stage is poorly immunogenic or that the antigen load is low.