PT  - JOURNAL ARTICLE
AU  - Y L Lin
AU  - N Lee
AU  - E C Chan
TI  - Determination of optimal liquid medium for enzyme expression by Helicobacter pylori.
AID  - 10.1136/jcp.49.10.818
DP  - 1996 Oct 01
TA  - Journal of Clinical Pathology
PG  - 818--820
VI  - 49
IP  - 10
4099  - http://jcp.bmj.com/content/49/10/818.short
4100  - http://jcp.bmj.com/content/49/10/818.full
SO  - J Clin Pathol1996 Oct 01; 49
AB  - AIMS: To determine the best medium for the growth and enzyme expression of Helicobacter pylori. METHODS: Twelve H pylori isolates from histologically confirmed infected patients were cultured on Brucella broth (BB), Brucella broth minus bisulphite (BLBB), and brain-heart infusion broth (BHIB), all supplemented with 5% (v/v) fetal calf serum. Growth rates and enzyme expressions of all H pylori isolates cultivated in these media were evaluated. RESULTS: Although both BLBB and BHIB supported good growth of H pylori under microaerophilic conditions, the total protein content of H pylori detected was much higher with BHIB cultivation. Measurement of the specific activities of urease, phospholipase C, and sphingomyelinase for 12 H pylori isolates cultivated in these media, showed that BHIB supported the highest expression of these enzymes. Although BLBB supported better growth of H pylori than BB, it did not increase enzyme expression. CONCLUSIONS: Cultivation of H pylori in BHIB is recommended for studies on the physiology, metabolism, and enzyme expression of the organism.