RT Journal Article
SR Electronic
T1 Decreased type V collagen expression in human decidual tissues of spontaneous abortion during early pregnancy.
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 44
OP 46
DO 10.1136/jcp.51.1.44
VO 51
IS 1
A1 Iwahashi, M
A1 Nakano, R
YR 1998
UL http://jcp.bmj.com/content/51/1/44.abstract
AB AIM: To provide some insight into the aetiology of spontaneous abortion, the contents of type V collagen was investigated in human decidual tissues in spontaneous abortion and normal pregnancy. METHODS: Collagens were extracted from decidual tissues in spontaneous abortion (n = 19) and normal pregnancy (n = 25). The different types of collagen alpha chains were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), stained with Coomassie brilliant blue, and measured by densitometry. The relative amounts of the alpha 1 (III) and alpha 1 (V) chains were calculated by dividing the band intensities of the alpha 1 (III) and alpha 1 (V) chains by that of the alpha 1 (I) chain. RESULTS: The ratio of the alpha 1 (V) chain to that of the alpha 1 (I) chain in decidual tissues in spontaneous abortion was significantly lower than that found in normal pregnancy (p < 0.05). CONCLUSIONS: These results suggest that type V collagen might play an important role in the maintenance of pregnancy and that decreased expression of this collagen could be associated with spontaneous abortion.