TY - JOUR T1 - Detection of fastidious bacteria in cardiac valves in cases of blood culture negative endocarditis JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 238 LP - 240 DO - 10.1136/jcp.54.3.238 VL - 54 IS - 3 AU - P Bruneval AU - J Choucair AU - F Paraf AU - J-P Casalta AU - D Raoult AU - F Scherchen AU - J-L Mainardi Y1 - 2001/03/01 UR - http://jcp.bmj.com/content/54/3/238.abstract N2 - The diagnosis of blood culture negative endocarditis is still a problem. Fastidious bacteria such as bartonella and coxiella are responsible for cases of blood culture negative endocarditis, the identification of which is mainly based on serological and DNA studies only available in specialised centres. Therefore, a routine technique is needed in surgical pathology laboratories to detect these bacteria in cardiac valve tissue sections. This report describes a staining technique, the Giménez stain, feasible and sensitive in detecting bartonella and coxiella in two cases of blood culture negative endocarditis. ER -