PT  - JOURNAL ARTICLE
AU  - K H Ko
AU  - S S Lee
AU  - J W Lawton
TI  - A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody.
AID  - 10.1136/jcp.52.10.770
DP  - 1999 Oct 01
TA  - Journal of Clinical Pathology
PG  - 770--772
VI  - 52
IP  - 10
4099  - http://jcp.bmj.com/content/52/10/770.short
4100  - http://jcp.bmj.com/content/52/10/770.full
SO  - J Clin Pathol1999 Oct 01; 52
AB  - AIM: To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum. METHODS: Latex bead coating was adjusted to give maximum sensitivity by attending to latex size, MPO to latex ratio for coupling, ratio of diluted serum to MPO-latex, reaction time and temperature for coupling, and reaction time for agglutination. Inhibition studies were performed using MPO, proteinase 3, bactericidal/permeability increasing protein, and lactoferrin. RESULTS: There was very good correlation between this test and the conventional anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positive in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated better with IgM anti-MPO than with IgG anti-MPO. CONCLUSIONS: MPO-LSAT is a simple diagnostic test that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseases.