PT  - JOURNAL ARTICLE
AU  - D Gancberg
AU  - A Di Leo
AU  - G Rouas
AU  - T Järvinen
AU  - A Verhest
AU  - J Isola
AU  - M J Piccart
AU  - D Larsimont
TI  - Reliability of the tissue microarray based FISH for evaluation of the HER-2 oncogene in breast carcinoma
DP  - 2002 Apr 01
TA  - Journal of Clinical Pathology
PG  - 315--317
VI  - 55
IP  - 4
4099  - http://jcp.bmj.com/content/55/4/315.short
4100  - http://jcp.bmj.com/content/55/4/315.full
SO  - J Clin Pathol2002 Apr 01; 55
AB  - Aims: Tumour tissue microarray allows the analysis of hundreds of tumour samples simultaneously on a single microscope slide. However, the extremely small tissue samples taken from the original tissue may not always be representative of the entire tumour. Methods: The reliability of this new technology was investigated by analysing HER-2 oncogene amplification by fluorescence in situ hybridisation (FISH) from representative slides of the whole tumour and small tissue core biopsies from 29 invasive breast tumours. Results: The tissue microarray method had high accuracy; in only one of 29 cases (3.4%; 95% confidence interval, 0% to 10%) were the results discordant with whole tumour analysis. Conclusion: Tumour microarray is a highly reliable method for analysing HER-2 oncogene amplification by FISH in human breast tumours.