RT Journal Article
SR Electronic
T1 Demystified… recombinant antibodies
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 912
OP 917
DO 10.1136/jcp.2003.014407
VO 57
IS 9
A1 K A Smith
A1 P N Nelson
A1 P Warren
A1 S J Astley
A1 P G Murray
A1 J Greenman
YR 2004
UL http://jcp.bmj.com/content/57/9/912.abstract
AB Recombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanised antibodies is given, together with details of the generation of antibody fragments—for example, single chain Fv fragments. Phage antibody fragments are fast becoming popular and can be generated by simple established methods of affinity enrichment from libraries derived from immune cells. Phage display methodology can also be used for the affinity enrichment of existing antibody fragments to provide a reagent with a higher affinity. Here, phage antibodies are demystified to provide a greater understanding of the potential of these reagents and to engage clinicians and biomedical scientists alike to think about potential applications in pathology and clinical settings.