RT Journal Article
SR Electronic
T1 Quality control in diagnostic molecular pathology in the Netherlands; proficiency testing for patient identification in tissue samples
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 717
OP 720
DO 10.1136/jcp.2003.011973
VO 57
IS 7
A1 F B J M Thunnissen
A1 M G J Tilanus
A1 M J L Ligtenberg
A1 P M Nederlof
A1 W N M Dinjens
A1 E Meulemans
A1 A J C Van den Brule
A1 C J M van Noesel
A1 W J F de Leeuw
A1 E Schuuring
YR 2004
UL http://jcp.bmj.com/content/57/7/717.abstract
AB Aims: To describe the evolution of proficiency testing for molecular diagnostic pathology with respect to determining unambiguously the patient identity of tissue samples by microsatellite analysis. Method: Four rounds of quality control exchanges of samples from different patients were sent with the purpose of identifying the correct origin of these samples. The samples were either paraffin wax embedded sections on glass, sections in tubes, or isolated DNA. Blinded samples were distributed to all participating laboratories. No restrictions to the method and short tandem repeat markers used for identification were imposed. Results: In four subsequent rounds the number of participating laboratories increased from three to 10. The numbers of samples tested increased in time from five to 12. The microsatellite markers used by the different laboratories showed little overlap. In the first three rounds, in which isolated DNA was provided, all samples were accurately classified irrespective of the microsatellite markers used. In the last round, which also included paraffin wax embedded sections, a small number of laboratories experienced problems, either with amplification or incorrect classification of a few samples. Conclusion: Proficiency testing was useful, and showed country wide high quality and correct identification of (patient) samples with molecular techniques for diagnostic purposes.