PT  - JOURNAL ARTICLE
AU  - S-H Zhang
AU  - W-M Cong
AU  - M-C Wu
TI  - Focal nodular hyperplasia with concomitant hepatocellular carcinoma: a case report and clonal analysis
AID  - 10.1136/jcp.2003.012823
DP  - 2004 May 01
TA  - Journal of Clinical Pathology
PG  - 556--559
VI  - 57
IP  - 5
4099  - http://jcp.bmj.com/content/57/5/556.short
4100  - http://jcp.bmj.com/content/57/5/556.full
SO  - J Clin Pathol2004 May 01; 57
AB  - This report describes a hepatocellular carcinoma (HCC) with concomitant focal nodular hyperplasia (FNH) in a 56 year old Chinese man. There were two well circumscribed tumours measuring 3 × 2.5 × 2 cm and 2 × 1.5 × 1.5 cm. The larger mass was grey and soft with a small area of bleeding and necrosis and an intact capsule. The smaller mass was yellow and had no capsule. Clonal analysis was carried out to clarify the relation between the HCC and the adjacent FNH. The clonal analysis was based on the methylation pattern of the polymorphic X chromosome linked androgen receptor gene (HUMARA). In FNH, after HpaII digestion, the allelic bands showed two well defined peaks. The intensity of the two peaks in the DNA from cirrhotic tissue did not differ significantly, consistent with a random pattern of X chromosome inactivation. However, in HCC, after HpaII digestion, the allelic bands differed significantly in intensity. Therefore, there was a typical polyclonal pattern of inactivation in FNH but the HCC was interpreted as being monoclonal.