TY - JOUR T1 - Molecular confirmation of invasive infection caused by <em>Chaetomium globosum</em> JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 334 LP - 334 VL - 58 IS - 3 AU - P J Paterson AU - S Seaton AU - T Yeghen AU - T D McHugh AU - J McLaughlin AU - A V Hoffbrand AU - C C Kibbler Y1 - 2005/03/01 UR - http://jcp.bmj.com/content/58/3/334.1.abstract N2 - Identification of the causative organism in invasive fungal infections is difficult. Accurate and reliable diagnostic methods are required in light of the increasing incidence of emerging fungal infections.1 We recently described a method for speciating fungi in formalin fixed, paraffin wax embedded tissue sections.2 DNA is extracted using a modification of the protocol for the TaKaRa DEXPAT™ kit (TaKaRa Biomedicals, Cambrex Biosciences, Wokingham, UK), with additional steps using lyticase and ethanol precipitation.2 Amplification of the DNA is performed using panfungal primers and a method based on that described by Einsele et al.3 Identification to species level can be achieved by Southern hybridisation with a probe that binds Aspergillus fumigatus, … ER -