TY - JOUR T1 - Hodgkin’s lymphoma associated T-cells exhibit a transcription factor profile consistent with distinct lymphoid compartments JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 1092 LP - 1097 DO - 10.1136/jcp.2006.044222 VL - 60 IS - 10 AU - Çiğdem Atayar AU - Anke van den Berg AU - Tjasso Blokzijl AU - Marcel Boot AU - Randy D Gascoyne AU - Lydia Visser AU - Sibrand Poppema Y1 - 2007/10/01 UR - http://jcp.bmj.com/content/60/10/1092.abstract N2 - Background: Hodgkin’s lymphoma (HL) is characterised by an ineffective immune response that is predominantly mediated by CD4+ T-cells. Aims: To analyse the expression of the key regulatory T-cell transcription factors (TFs) in the T-cells of HL involved tissues in order to assess the nature of the TH immune response in HL. Methods and results: By immunohistochemistry, GATA3 was strongly and T-bet exclusively expressed in a subset of interfollicular lymphocytes in the reactive lymphoid tissues. In classical HL (CHL), which is generally located in the interfollicular zones, a predominance of T-bet+ T-cells and lesser amounts of GATA3+ and c-Maf+ T-cells was found, concordant with the pattern of the normal interfollicular compartment. In reactive lymphoid tissues, c-Maf was observed mostly in T-lymphocytes within the germinal centres (GCs). Nodular lymphocyte predominance type of Hodgkin’s lymphoma (NLPHL) and progressively transformed germinal centres cases, showed a majority of c-Maf+ T-cells, consistent with the pattern in normal GCs. NLPHL cases uniformly showed c-Maf+/CD57+ T-cell rosettes around the neoplastic cells; these rosettes were absent in “paragranuloma-type” T-cell/histiocyte rich B-cell lymphoma. Conclusions: T-cell TF expression profiles of the reactive T-cells in both subtypes of HL are in accordance with the expression profile observed in the distinct lymphoid compartments. ER -