PT  - JOURNAL ARTICLE
AU  - Q-F Xia
AU  - Z-G Tu
AU  - J-B Liu
AU  - X Qin
AU  - S-Y Qian
AU  - P Li
TI  - Detection of PCR products using self-reporting duplex mutation primers
AID  - 10.1136/jcp.2009.064402
DP  - 2009 Nov 01
TA  - Journal of Clinical Pathology
PG  - 1046--1048
VI  - 62
IP  - 11
4099  - http://jcp.bmj.com/content/62/11/1046.short
4100  - http://jcp.bmj.com/content/62/11/1046.full
SO  - J Clin Pathol2009 Nov 01; 62
AB  - A novel oligonucleotide probe, with duplex mutation primers, which comprised a normal reverse primer labelled with a fluorophore at its 5′-end and a single-base mismatched complementary oligonucleotide labelled with a quencher at its 3′-end, was designed. Its application in the detection of hepatitis B virus (HBV) DNA of serum was investigated; results were compared with those obtained using a commercial TaqMan kit. There was a good linear correlation in the range of 102∼107 copies/ml (r2 = 0.999) with the method. Intra-experimental coefficients of variation (CVs) were 0.70%∼7.80%, and inter-experimental CVs were 0.78%∼9.02%, respectively. There was no significant difference of HBV genome number tested by the two methods (p<0.05) in 132 hepatitis B patients; HBV DNA was not detected in any serum samples of 20 healthy volunteers by the two methods.