TY - JOUR T1 - Chronic atrial fibrillation associated with somatic mitochondrial DNA mutations in human atrial tissue JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 948 LP - 950 DO - 10.1136/jcp.2007.047134 VL - 60 IS - 8 AU - Hyung-Wook Park AU - Youngkeun Ahn AU - Myung-Ho Jeong AU - Jeong-Gwan Cho AU - Jong-Chun Park AU - Jung-Chaee Kang AU - Myung-Geun Shin AU - Jong-Hee Shin AU - Soon-Pal Suh AU - Dong-Wook Ryang AU - Nam-Ho Kim AU - Jong-Bum Choi AU - Hye-Ran Kim Y1 - 2007/08/01 UR - http://jcp.bmj.com/content/60/8/948.abstract N2 - Somatically acquired mitochondrial DNA (mtDNA) mutations have been linked to aging, degenerative diseases, cancer and organ dysfunction. mtDNA alterations were investigated in matched atrial tissues and blood samples from four patients with chronic atrial fibrillation (cAF) and two matched patients without cAF. Nine novel mtDNA mutations were observed in mtDNA control and coding region. Interestingly, two patients with cAF had tissue-specific length heteroplasmic mutations from nucleotide 16184 to 16193 of the polyC tract and CA repeats starting at nucleotide 514. A 9 bp deletion (nucleotides 8271–8279) in the mtDNA COII gene was only found in tissues and blood cells from two patients with cAF. In patients with cAF, mtDNA mutations, including small deletions and tissue-specific length heteroplasmic mutations, occurred in both mtDNA control and coding regions. These findings strongly suggest that mtDNA mutations may play a crucial role in atrial dysfunction in patients with cAF. Mitochondria are both the power plant of human cells and the target of reactive oxygen species and free radicals. With ageing and excess (ROS) stress, free radicals and (ROS) can overwhelm the antioxidant system and result in damage to cellular constituents such as lipids, proteins and DNA.1 A few papers have reported that mitochondrial DNA (mtDNA) deletion mutation in human atrial tissue is associated with chronic atrial fibrillation (cAF).2,3 However, these studies only checked mtDNA deletion in atrial tissue and did not examine the corresponding blood samples. Therefore, we analysed the mtDNA control region and cytochrome c oxidase (CO) I, COII, COIII, ATPase 6 and cytochrome b (Cytb) genes in matched atrial tissues and blood samples from patients with cAF and in two matched patients with normal sinus rhythm and no history of cAF. Atrial tissue samples and corresponding blood samples were taken from four patients with cAF and from two matched … ER -