RT Journal Article
SR Electronic
T1 Analysis of microsatellite instability in colorectal carcinoma by microfluidic-based chip electrophoresis
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 850
OP 852
DO 10.1136/jcp.2008.056994
VO 62
IS 9
A1 M Odenthal
A1 N Barta
A1 D Lohfink
A1 U Drebber
A1 F Schulze
A1 H P Dienes
A1 S E Baldus
YR 2009
UL http://jcp.bmj.com/content/62/9/850.abstract
AB Microsatellite analysis is an important tool in clinical research and molecular diagnostics because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10–15% of colorectal, gastric and endometrial carcinomas are associated with MSI, and this has an impact on clinical prognosis. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250, recommended by the Bethesda guidelines, were analysed by microfluidic-based on-chip electrophoresis in 40 cases of colon carcinoma with known MSI status. In all cases, microfluidic separation of the PCR amplicons resulted in highly resolved, distinct patterns of each of the five microsatellite loci. Detection of MSI could be demonstrated by microsatellite-loci-associated, well-defined deviations in the electropherogram profiles of tumour and non-tumour material, and confirmed the classification of MSI cases performed by conventional technology. In conclusion, microfluidic chip technology is a simple and reliable approach for MSI detection that allows label-free and very fast analysis of microsatellite amplicons.