RT Journal Article
SR Electronic
T1 Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 702
OP 707
DO 10.1136/jcp.2010.078717
VO 63
IS 8
A1 Shantibhusan Senapati
A1 Samuel B Ho
A1 Poonam Sharma
A1 Srustidhar Das
A1 Subhankar Chakraborty
A1 Sukhwinder Kaur
A1 Gloria Niehans
A1 Surinder K Batra
YR 2010
UL http://jcp.bmj.com/content/63/8/702.abstract
AB Aim To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon.Methods Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR.Results MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p<0.0001) and ischaemic colitis (p=0.003). Similarly, MUC17 expression was decreased in hyperplastic polyps (p=0.0003), tubular and tubulovillous adenomas (p<0.0001) and colon cancers (p<0.0001). Furthermore, of eight different colon cancer cell lines, MUC17 expression was only detected in LS174T and LS180 cells.Conclusion Results indicate that the potential protective effects of this membrane-bound mucin are primarily or secondarily diminished in inflammatory and neoplastic conditions. Further research is needed to determine the specific role of MUC17 in the pathogenesis of these conditions.