TY - JOUR T1 - Allele-specific wild-type blocker quantitative PCR for highly sensitive detection of rare JAK2 p.V617F point mutation in primary myelofibrosis as an appropriate tool for the monitoring of molecular remission following therapy JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 370 LP - 372 DO - 10.1136/jcp.2009.069773 VL - 63 IS - 4 AU - Udo Siebolts AU - Thoralf Lange AU - Dietger Niederwieser AU - Claudia Wickenhauser Y1 - 2010/04/01 UR - http://jcp.bmj.com/content/63/4/370.abstract N2 - Screening of JAK2 V617F point mutation becomes more and more important in monitoring of JAK2 positive MPN following stem cell transplantation. In an attempt to achieve the required high sensitivity (1:105), specifity and robustness we created an approach applicable on bone marrow biopsies where we adapted the principle of wild-type blocker PCR with allele-specific Q-PCR. The significance of the assay was demonstrated on a retrospective series of sequential bone marrow biopsies as diagnosis of molecular relapse now preceded the diagnosis of clinical relapse by far. This method offers the urgently needed tool for a systematic molecular analysis of sequential biopsies in the course of stem cell transplantation to develop guidelines for the management of these patients. ER -