RT Journal Article
SR Electronic
T1 Epidermal growth factor receptor mutations in malignant pleural and peritoneal mesothelioma
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 522
OP 527
DO 10.1136/jclinpath-2011-200631
VO 65
IS 6
A1 Yasunori Enomoto
A1 Takahiko Kasai
A1 Maiko Takeda
A1 Masato Takano
A1 Kohei Morita
A1 Eiji Kadota
A1 Norishige Iizuka
A1 Hiroshi Maruyama
A1 Joji Haratake
A1 Yu Kojima
A1 Naoya Ikeda
A1 Naoki Inatsugi
A1 Akitaka Nonomura
YR 2012
UL http://jcp.bmj.com/content/65/6/522.abstract
AB Background Epidermal growth factor receptor (EGFR) gene mutation at the kinase domain and EGFR gene amplification are reported to be predictors of the response to EGFR tyrosine kinase inhibitors in lung cancer cases. In malignant mesothelioma (MM), the role of EGFR is less clear.Methods Thirty-eight MM specimens were submitted to EGFR mutation evaluation, and compared with the results of immunohistochemical staining and fluorescence in situ hybridization (FISH) analysis. DNA was extracted from paraffin blocks and PCR was performed to amplify exon regions 18–21 of the EGFR gene. Direct sequencing of the purified PCR products was performed.Results Five EGFR missense mutations were detected in six of the 38 patients (16%); two of these mutations were novel, two were originally detected in non-small cell lung carcinoma, and one resembled a location previously noted for malignant peritoneal mesothelioma.Conclusion As far as the authors are aware there has been no report of the EGFR mutation of MM in Japanese cases, but in this study EGFR missense mutations were detected in some cases. EGFR mutation results were not related to immunohistochemical and FISH analysis.