TY - JOUR T1 - Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 660 LP - 662 DO - 10.1136/jclinpath-2012-200716 VL - 65 IS - 7 AU - Berit Maria Müller AU - Jan C Brase AU - Franziska Haufe AU - Karsten E Weber AU - Jan Budzies AU - Christoph Petry AU - Judith Prinzler AU - Ralf Kronenwett AU - Manfred Dietel AU - Carsten Denkert Y1 - 2012/07/01 UR - http://jcp.bmj.com/content/65/7/660.abstract N2 - Aim This study compared the perfomance of the RNA-based EndoPredict multigene test on core biopsies and surgical breast cancer specimens and analysed the influence of biopsy-induced tissue injuries on the test result.Methods 80 formalin-fixed paraffin-embedded samples comprising paired biopsies and surgical specimens from 40 ER-positive, HER2-negative patients were evaluated. Total RNA was extracted and the EndoPredict score was determined.Results RNA yield was considerably lower in core biopsies, but sufficient to measure the assay in all samples. The EndoPredict score was highly correlated between paired samples (Pearson r=0.92), with an excellent concordance of classification into a low or high risk of metastasis (overall agreement 95%).Conclusions The measurements are comparable between core biopsies and surgical sections, which suggest that the EndoPredict assay can be performed on core biopsy tissue. Inflammatory changes induced by presurgical biopsies had no significant effect on the RNA-based risk assessment in surgical specimens. ER -