RT Journal Article
SR Electronic
T1 Epigenomic profiling in polycythaemia vera and essential thrombocythaemia shows low levels of aberrant DNA methylation
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 1010
OP 1013
DO 10.1136/jclinpath-2011-200175
VO 64
IS 11
A1 S Barrio
A1 M Gallardo
A1 E Albizua
A1 A Jimenez
A1 I Rapado
A1 R Ayala
A1 F Gilsanz
A1 J I Martin-Subero
A1 J Martinez-Lopez
YR 2011
UL http://jcp.bmj.com/content/64/11/1010.abstract
AB Aims The purpose of this study was to compare the DNA-methylation signature in classic chronic Philadelphia negative myeloproliferative neoplasms (MPN), polycythaemia vera (PV) and essential thrombocythaemia (ET), in order to obtain a global insight into DNA-methylation changes associated with these malignancies.Methods Thirty-five MPN samples from 11 ET JAK2 V617F, 12 ET JAK2 wild type (WT) and 12 PV JAK2 V617F patients as well as 12 from healthy donors were analysed. DNA samples extracted from whole peripheral blood were hybridised to the ‘HumanMethylation27 DNA Analysis BeadChip.’Results All groups showed a very homogeneous methylation pattern. Only the ZNF577 gene showed a differential methylation profile between PV JAK2 V617F positive and controls. This aberrant methylation was correlated with a differential gene expression of ZNF577. No aberrant hypermethylation was found in the SOCS-1 and SOCS-3 genes.Conclusions According to our results, an aberrant methylation pattern does not seem to play a crucial role in MPN pathogenesis; nor does it justify phenotypical differences between PV and ET.