PT - JOURNAL ARTICLE AU - Briggs, Carol AU - Longair, Ian AU - Kumar, Punamar AU - Singh, Deepak AU - Machin, Samuel J TI - Performance evaluation of the Sysmex haematology XN modular system AID - 10.1136/jclinpath-2012-200930 DP - 2012 Nov 01 TA - Journal of Clinical Pathology PG - 1024--1030 VI - 65 IP - 11 4099 - http://jcp.bmj.com/content/65/11/1024.short 4100 - http://jcp.bmj.com/content/65/11/1024.full SO - J Clin Pathol2012 Nov 01; 65 AB - Background The Sysmex XN haematology instrument performs automatic reflex testing, depending on sample results. A nucleated red blood cell (NRBC) count is provided on all samples. The instrument has a smaller footprint (34%) than previous Sysmex XE analysers. Methods An evaluation comparing all results to the Sysmex XE-2100 and manual microscopic differential and morphology (n=390) was performed followed by a workflow study of 1000 samples to compare speed of operation and number of blood films reviews required from both systems. Results The new features on the instrument are: (1) white cell and NRBC channel, all samples include the NRBC count; (2) white cell precursor channel: false positive flags for blasts, abnormal lymphocytes and atypical lymphocytes are reduced significantly without a statistical increase of false negatives; (3) low white cell count mode: suggested setting of <0.5×109/l. An extended count is more precise and provides an accurate differential. Fluorescent platelet count is performed in a dedicated channel. If the red cell or platelet size histograms are abnormal or if the platelet count is low, then a fluorescent platelet count is automatically performed. Good correlation with the XE-2100 and manual differential was found and the improved results compared to the reference flow cytometric analysis for platelet counts, especially below 30×109/l (XE-2100, R2=0.500; XN, R2=0.875). Conclusion The XN showed reduced sample turnaround time of 10% and reduced number of blood films for examination, 49% less than the XE-2100 without loss of sensitivity with more precise and accurate results on low cell counts.