PT  - JOURNAL ARTICLE
AU  - David Manson-Bahr
AU  - Richard Ball
AU  - Gunes Gundem
AU  - Krishna Sethia
AU  - Robert Mills
AU  - Mark Rochester
AU  - Victoria Goody
AU  - Elizabeth Anderson
AU  - Sarah O'Meara
AU  - Marcus Flather
AU  - Matthew Keeling
AU  - Marcelino Yazbek-Hanna
AU  - Rachel Hurst
AU  - Helen Curley
AU  - Jeremy Clark
AU  - Daniel S Brewer
AU  - Ultan McDermott
AU  - Colin Cooper
TI  - Mutation detection in formalin-fixed prostate cancer biopsies taken at the time of diagnosis using next-generation DNA sequencing
AID  - 10.1136/jclinpath-2014-202754
DP  - 2015 Mar 01
TA  - Journal of Clinical Pathology
PG  - 212--217
VI  - 68
IP  - 3
4099  - http://jcp.bmj.com/content/68/3/212.short
4100  - http://jcp.bmj.com/content/68/3/212.full
SO  - J Clin Pathol2015 Mar 01; 68
AB  - Aims Assessing whether next-generation DNA sequencing (NGS) can be used to screen prostate cancer for multiple gene alterations in men routinely diagnosed with this disease and/or who are entered into clinical trials. Previous studies are limited and have reported only low success rates. Methods We marked areas of cancer on H&E-stained sections from formalin-fixed needle biopsies, and used these as templates to dissect cancer-rich tissue from adjacent unstained sections. DNA was prepared using a Qiagen protocol modified to maximise DNA yield. The DNA was screened simultaneously for mutations in 365 cancer-related genes using an Illumina HiSeq 2000 NGS platform. Results From 63 prostate cancers examined, 59 (94%) of the samples yielded at least 30 ng of DNA, the minimum amount of DNA considered suitable for NGS analysis. Patients in the D'Amico high-risk group yielded an average of 1033 ng, intermediate-risk patients 401 ng, and low-risk patients 97 ng. NGS of eight samples selected from high-risk and intermediate-risk groups gave a median exon read depth of 962 and detected TMPRRS2-ERG fusions, as well as a variety of mutations including those in the SPOP, TP53, ATM, MEN1, NBPF10, NCOR2, PIK3CB and MAP2K5 (MEK5) genes. Conclusions Using the methods presented here, NGS technologies can be used to screen a high proportion of patients with prostate cancer for mutations in cancer-related genes in tissue samples opening up its general use in the context of clinical trials or routine diagnosis.