TY - JOUR T1 - A novel semi-automated in situ hybridisation protocol for microRNA detection in paraffin embedded tissue sections JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 661 LP - 664 DO - 10.1136/jclinpath-2015-203005 VL - 68 IS - 8 AU - Ambra Vittoria Gualeni AU - Chiara Costanza Volpi AU - Antonino Carbone AU - Annunziata Gloghini Y1 - 2015/08/01 UR - http://jcp.bmj.com/content/68/8/661.abstract N2 - MicroRNAs (miRNAs) are small non-coding RNAs that modulate gene expression by binding to complementary sequences on target messenger RNA transcripts. In situ hybridisation (ISH) methods have been applied to the study of miRNA in tissue samples in order to understand which is the source of the miRNA of interest. In this paper, the authors describe a novel semi-automated bright field ISH method to visualise miRNAs in formalin fixed paraffin embedded tissue sections. The relevance of this work resides in the use of 3,3′-diaminobenzidine and peroxidase as the detection method, which provides a good defined deposition within tissues This method, which reveals the cells of origin of specific miRNAs, will enable investigators to further explore the biological role of miRNAs. ER -