RT Journal Article
SR Electronic
T1 The utility of a novel antibody in the pathological diagnosis of pancreatic acinar cell carcinoma
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP jclinpath-2011-200442
DO 10.1136/jclinpath-2011-200442
A1 Makiko Yasumoto
A1 Masato Hamabashiri
A1 Jun Akiba
A1 Sachiko Ogasawara
A1 Yoshiki Naito
A1 Tomoki Taira
A1 Masamichi Nakayama
A1 Aya Daicho
A1 Fumio Yamasaki
A1 Kazuhide Shimamatsu
A1 Yusuke Ishida
A1 Ryohei Kaji
A1 Yoshinobu Okabe
A1 Osamu Nakashima
A1 Koichi Ohshima
A1 Manabu Nakashima
A1 Michio Sata
A1 Hirohisa Yano
YR 2012
UL http://jcp.bmj.com/content/early/2012/01/06/jclinpath-2011-200442.abstract
AB Aims Acinar cell carcinomas (ACCs) are rare tumours of the exocrine pancreas accounting for about 1–2% of all pancreatic neoplasms in adults. It is therefore difficult to come across a large number of ACC cases in a single medical institution, and only a few serial studies have been published. Since ACCs present a wide variety of morphological patterns, immunohistochemical analysis is useful. In this study, the authors established a novel monoclonal antibody 2P-1-2-1 by means of a subtractive immunisation method.Methods Immunohistochemical staining was performed using 50 primary pancreatic tumors, including 7 ACCs, 7 neuroendocrine tumours (NETs), 5 solid-pseudopapillary neoplasms (SPNs), and 31 ductal carcinomas and organs other than the pancreas.Results Non-neoplastic acinar cells were stained diffusely, but epithelial cells of the pancreatic duct and the islets of Langerhans were not stained. In pancreatic tumours, all the seven ACCs were diffusely positive for the 2P-1-2-1 antibody. However, no positive staining was found in other pancreatic tumours including NETs, SPNs and ductal adenocarcinomas. The sensitivity and specificity of the 2P-1-2-1 antibody for ACCs were both 100%. In other organs studied, positive staining was observed only in the ectopic pancreas.Conclusions It was shown that the 2P-1-2-1 antibody specifically stained the pancreatic acinar cells and tumours of acinar cell origin, such as ACCs. Although it remains unclear at this time to which proteins the monoclonal antibody 2P-1-2-1 is directed, it is suggested to be useful for the pathological diagnosis of ACCs and for the exclusion of other pancreatic tumours.