PT  - JOURNAL ARTICLE
AU  - Richard Colling
AU  - Lai Mun Wang
AU  - Elizabeth Soilleux
TI  - Automated PCR detection of <em>BRAF</em> mutations in colorectal adenocarcinoma: a diagnostic test accuracy study
AID  - 10.1136/jclinpath-2015-203345
DP  - 2016 May 01
TA  - Journal of Clinical Pathology
PG  - 398--402
VI  - 69
IP  - 5
4099  - http://jcp.bmj.com/content/69/5/398.short
4100  - http://jcp.bmj.com/content/69/5/398.full
SO  - J Clin Pathol2016 May 01; 69
AB  - Background Testing for BRAF mutations in colorectal carcinoma (CRC) is important in the screening pathway for Lynch syndrome and is of prognostic value to guide management. This is a diagnostic accuracy study of the Idylla system, a novel and automated alternative PCR system.Methods 100 consecutive formalin-fixed, paraffin-embedded CRC resection cases were tested for BRAF mutations using the Idylla automated platform and compared with standard (Cobas) PCR.Results The sensitivity of the Idylla BRAF test was 100% and the specificity was 96%. Only one discordant Idylla positive/standard PCR negative result occurred and on Droplet Digital PCR demonstrated a mutation not identified by traditional PCR in this case.Conclusion This study has validated the Idylla system for BRAF testing in CRC and demonstrated a possibly greater sensitivity, in addition to cost effectiveness and shorter turnaround time, when compared with standard PCR.