@article {Zhang1100, author = {Xi Zhang and Yunxia Wan and Roberto Chata and Anthony Brazzale and John J Atherton and Karam Kostner and Goce Dimeski and Chamindie Punyadeera}, title = {A pilot study to demonstrate diagnostic potential of galectin-3 levels in saliva}, volume = {69}, number = {12}, pages = {1100--1104}, year = {2016}, doi = {10.1136/jclinpath-2016-203631}, publisher = {BMJ Publishing Group}, abstract = {Aim Heart failure (HF) affects millions of older individuals in both developed and low/middle-income countries. Serum galectin-3 levels have been shown to have prognostic value. However, its use as a diagnostic biomarker has not been explored. The aim was to establish a saliva galectin-3 reference range and to demonstrate the potential diagnostic utility of salivary and serum galectin-3 levels in assessing HF.Methods Blood and saliva samples were collected from age-matched healthy controls (n=51) and patients with HF (n=63). Customised immunoassays were developed to quantify salivary galectin-3 levels. The diagnostic performances of these assays were evaluated by receiver operator characteristic (ROC) curves analysis.Results The galectin-3 concentrations were significantly elevated in saliva and serum samples of patients with HF compared with controls (p\<0.001 and p\<0.0001, respectively). Using ROC curve analysis, both serum and salivary galectin-3 gave area under the curve (AUC)=0.86 and AUC=0.73, respectively. There was also a significant correlation (r=0.4, p\<0.01) between serum and salivary galectin-3 levels.Conclusions For the first time, we have quantified galectin-3 levels in human saliva and have demonstrated potential clinical utility in diagnosing HF. Further, larger multicentre clinical trials are needed before salivary galectin-3 levels can be implemented in a clinical setting.}, issn = {0021-9746}, URL = {https://jcp.bmj.com/content/69/12/1100}, eprint = {https://jcp.bmj.com/content/69/12/1100.full.pdf}, journal = {Journal of Clinical Pathology} }