TY - JOUR T1 - Microsatellite instability evaluation by automated microfluidic electrophoresis: an update JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 90 LP - 91 DO - 10.1136/jclinpath-2016-204200 VL - 70 IS - 1 AU - Francesco Pepe AU - Riccardo Smeraglio AU - Davide Vacirca AU - Umberto Malapelle AU - Massimo Barberis AU - Giancarlo Troncone Y1 - 2017/01/01 UR - http://jcp.bmj.com/content/70/1/90.2.abstract N2 - A significant proportion (∼15%) of colorectal cancer (CRC), either sporadic or arising in the setting of the hereditary non-polyposis colorectal carcinoma syndrome, features microsatellite instability (MSI). Five MSI loci, either mononucleotide or dinucleotide repeats (Bat25, Bat26, D2S123, D5S346 and D17S250), are included in the Bethesda panel and capillary electrophoresis represents the usual gold standard technique.1 Samples are classified as MSI-low (L) if only one locus is abnormal or as MSI-high (H) if alterations extend to two or more loci. In a study published in 2009 in the Journal of Clinical Pathology, Odenthal et al2 validated, on the 2100 Bioanalyzer (Agilent … ER -