TY - JOUR T1 - Cushing's syndrome: a practical approach to diagnosis and differential diagnoses JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 350 LP - 359 DO - 10.1136/jclinpath-2016-203933 VL - 70 IS - 4 AU - Joseph M Pappachan AU - Christian Hariman AU - Mahamood Edavalath AU - Julian Waldron AU - Fahmy W Hanna Y1 - 2017/04/01 UR - http://jcp.bmj.com/content/70/4/350.abstract N2 - Diagnosis of Cushing's syndrome (CS) and identification of the aetiology of hypercortisolism can be challenging. The Endocrine Society clinical practice guidelines recommends one of the four tests for initial screening of CS, namely, urinary-free cortisol, late night salivary cortisol, overnight dexamethasone suppression test or a longer low-dose dexamethasone suppression test, for 48 hours. Confirmation and localisation of CS requires additional biochemical and radiological tests. Radiological evaluation involves different imaging modalities including MRI with or without different radio-nuclear imaging techniques. Invasive testing such as bilateral inferior petrosal sinus sampling may be necessary in some patients for accurate localisation of the cause for hypercortisolism. This best practice review discusses a practical approach for the diagnostic evaluation of CS with a brief discussion on differential diagnoses, and cyclical CS, to enhance the skills of clinicians and laboratory personnel. ER -