PT  - JOURNAL ARTICLE
AU  - Min-Kyung Yeo
AU  - Hee Eun Kim
AU  - Sung Hun Kim
AU  - Byung Joo Chae
AU  - Byung Joo Song
AU  - Ahwon Lee
TI  - Clinical usefulness of the free web-based image analysis application ImmunoRatio for assessment of Ki-67 labelling index in breast cancer
AID  - 10.1136/jclinpath-2016-204162
DP  - 2017 Aug 01
TA  - Journal of Clinical Pathology
PG  - 715--719
VI  - 70
IP  - 8
4099  - http://jcp.bmj.com/content/70/8/715.short
4100  - http://jcp.bmj.com/content/70/8/715.full
SO  - J Clin Pathol2017 Aug 01; 70
AB  - Aims Ki-67 is a prognostic marker in breast cancer; however, the use of the Ki-67 labelling index (LI) in clinical practice requires a consistent and easily accessible scoring method. The present study evaluated the use of the free internet-based image analysis program ImmunoRatio to score Ki-67 LI in breast cancer in comparison with manual counting.Methods Ki-67 immunohistochemical detection was performed in 577 breast cancer cases, and the Ki-67 LI was determined by ImmunoRatio and manual counting.Results The Ki-67 LI determined by ImmunoRatio correlated well with that obtained by manual counting. The concordance rate between ImmunoRatio and manual counting was excellent (κ coefficient of 0.881) at a Ki-67 LI cut-off value of 20%. Cases with high Ki-67 LI by ImmunoRatio were associated with poor overall survival, in particular in the hormone receptor positive group.Conclusions The web-based automated image analysis program ImmunoRatio is an attractive alternative to manual counting to determine the Ki-67 LI in breast cancer.