TY - JOUR T1 - Multiplex digital colour-coded barcode technology on RNA extracted from routine cytological samples of patients with non-small cell lung cancer: pilot study JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 803 LP - 806 DO - 10.1136/jclinpath-2017-204373 VL - 70 IS - 9 AU - Roberta Sgariglia AU - Pasquale Pisapia AU - Mariantonia Nacchio AU - Caterina De Luca AU - Francesco Pepe AU - Maria Russo AU - Claudio Bellevicine AU - Giancarlo Troncone AU - Umberto Malapelle Y1 - 2017/09/01 UR - http://jcp.bmj.com/content/70/9/803.abstract N2 - In the advanced stages of non-small cell lung cancer (NSCLC), molecular testing is often performed on archival cytological smears. The nCounter system (NanoString Technologies) is a new promising multiplex digital colour-coded barcode technology. However, its feasibility to evaluate the RNA expression of clinical relevant biomarkers on routine cytological smears is still uncertain. To this end, RNA was extracted from 12 NSCLC routine stained cytological smears, and nCounter analysis performed by using a 48-gene panel. Overall, 11/12 (92%) of the smears were adequate for the secondary analysis, fulfilling the quality check parameter analysis of nSolver software. This pilot study shows that RNA nCounter analysis is feasible on routine cytological smears preparing the field for the implementation of this technology in the routine setting. ER -