PT  - JOURNAL ARTICLE
AU  - Heba W Z Khella
AU  - Nicole Daniel
AU  - Leza Youssef
AU  - Andreas Scorilas
AU  - Roy Nofech-Mozes
AU  - Lorna Mirham
AU  - Sergey N Krylov
AU  - Evi Liandeau
AU  - Adriana Krizova
AU  - Antonio Finelli
AU  - Yufeng Cheng
AU  - George M Yousef
TI  - miR-10b is a prognostic marker in clear cell renal cell carcinoma
AID  - 10.1136/jclinpath-2017-204341
DP  - 2017 Oct 01
TA  - Journal of Clinical Pathology
PG  - 854--859
VI  - 70
IP  - 10
4099  - http://jcp.bmj.com/content/70/10/854.short
4100  - http://jcp.bmj.com/content/70/10/854.full
SO  - J Clin Pathol2017 Oct 01; 70
AB  - Aims Clear cell renal cell carcinoma (ccRCC) is the most common adult kidney cancer. It is an aggressive tumour with unpredictable outcome. The currently used clinical parameters are not always accurate for predicting disease behaviour. miR-10b is dysregulated in different malignancies including RCC.Methods We assessed the clinical utility of miR-10b as a prognostic marker in 250 patients with primary ccRCC. We examined the correlation between miR-10b and clinicopathological parameters. We compared miR-10b expression among different RCC subtypes and normal kidney tissue.Results We observed a stepwise decrease of miR-10b expression from normal kidney to primary ccRCC and a further decrease from primary to metastatic RCC. miR-10b expression was significantly lower in stages III/IV compared with stages I/II (p=0.038). Using a binary cut-off, miR-10b-positive patients had significantly longer disease-free survival (HR=0.47, CI 0.28 to 0.79, p=0.004). In the subgroup of patients with tumour size >4 cm, higher miR-10b expression was associated with significant longer disease-free and overall survival (p=0.001 and p=0.036, respectively). miR-10b was significantly downregulated in ccRCC compared with normal kidney (p<0.0001), and oncocytoma (p=0.031). It was also downregulated in chromophobe RCC. In addition, we identified a number of miR-10b-predicted targets and pathways that are involved in tumourigenesis.Conclusions Our data point to miR-10b as a promising prognostic marker in ccRCC with potential therapeutic applications.