@article {Barbosajclinpath-2018-205024, author = {Jakeline Ribeiro Barbosa and Vanessa Faria Cortes and Moyra Machado Portilho and Juliana Cust{\'o}dio Miguel and Vanessa Alves Marques and Cristianne Sousa Bezerra and Natalia Vasconcelos de Souza and Danielle Malta Lima and Jeov{\'a} Keny Baima Colares and Lia Laura Lewis-Ximenez and Elisabeth Lampe and Livia Melo Villar}, title = {Performance of point of care assays for hepatitis B and C viruses in chronic kidney disease patients}, elocation-id = {jclinpath-2018-205024}, year = {2018}, doi = {10.1136/jclinpath-2018-205024}, publisher = {BMJ Publishing Group}, abstract = {Aims Point of care testing (POCT) has been used for hepatitis B and C diagnosis in general population, but little is known about the influence of clinical conditions in the accuracy of these assays. This study aims to evaluate the performance of POCTs for detection of hepatitis B virus surface antigen (HBsAg) and antibodies to Hepatitis C Virus (anti-HCV) in Chronic Kidney Disease (CKD) patients.Methods A total of 286 subjects were included in this study. HBsAg and anti-HCV were detected using commercial EIAs and four POCTs: HBsAg (WAMA Imuno-R{\'a}pido HBsAg and VIKIA HBsAg) and anti-HCV (DOLES HCV teste r{\'a}pido and WAMA Imuno-R{\'a}pido anti-HCV) in serum and whole blood.Results Using EIA, HBsAg and anti-HCV prevalence was 4.5\% and 16.1\% in CKD patients. HBsAg and anti-HCV POCTs had sensitivities from 92.3\% to 100\% and 84.8\% to 89.1\% while specificities were 99.3\% to 100\% and 99.2\% to 99.6\%, respectively. POCT using serum samples performed well compared with whole blood samples and true positive samples of POCTs had high optical density to cut-off (OD/CO) values compared with EIA.Conclusions This study demonstrates good performance of HBsAg and anti-HCV POCTs in CKD patients, especially in serum samples indicating low interference of this disease in the performance of these assays. POCTs could be an important tool for HBV and HCV screening in high-risk populations.}, issn = {0021-9746}, URL = {https://jcp.bmj.com/content/early/2018/05/05/jclinpath-2018-205024}, eprint = {https://jcp.bmj.com/content/early/2018/05/05/jclinpath-2018-205024.full.pdf}, journal = {Journal of Clinical Pathology} }