RT Journal Article
SR Electronic
T1 Identification of IgG3-specific epitope that remedies problem in diagnostic IgG subclass determination due to human genetic variation
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 559
OP 561
DO 10.1136/jclinpath-2018-205001
VO 71
IS 6
A1 Howie, Heather L
A1 Wang, Xiaohong
A1 Kapp, Linda
A1 Lebedev, Jenna N
A1 Zimring, James C
YR 2018
UL http://jcp.bmj.com/content/71/6/559.abstract
AB There are four subtypes of human IgG with different effector functions. Quantifying the relative amount of each IgG subtype is important for laboratory diagnosis in multiple settings. However, in an evolving landscape of the appreciation of human variability and the need for precision/personalised laboratory diagnosis, it has also been shown that there are numerous natural variants of IgG subtypes, with at least 29 having been described. It has recently been reported that commercially available polyclonal antisera to IgG3 cross react with variants of other IgG subtypes, while available monoclonal anti-IgG3 have a blind-spot for the IgG3-04 variant. Herein, we report that IgG3-04 contains an epitope in common with all known IgG3 variants and absent in other subtypes. A novel monoclonal anti-IgG3 is described that is specific to IgG3 but without any blind-spots for known IgG3 variants, providing a remedy to the problem of genetic variability of IgG3.