PT  - JOURNAL ARTICLE
AU  - Keegan, Alissa
AU  - Charest, Karry
AU  - Schmidt, Ryan
AU  - Briggs, Debra
AU  - Deangelo, Daniel J
AU  - Li, Betty
AU  - Morgan, Elizabeth A
AU  - Pozdnyakova, Olga
TI  - Flow cytometric minimal residual disease assessment of peripheral blood in acute lymphoblastic leukaemia patients has potential for early detection of relapsed extramedullary disease
AID  - 10.1136/jclinpath-2017-204828
DP  - 2018 Jul 01
TA  - Journal of Clinical Pathology
PG  - 653--658
VI  - 71
IP  - 7
4099  - http://jcp.bmj.com/content/71/7/653.short
4100  - http://jcp.bmj.com/content/71/7/653.full
SO  - J Clin Pathol2018 Jul 01; 71
AB  - Objectives To evaluate peripheral blood (PB) for minimal residual disease (MRD) assessment in adults with acute lymphoblastic leukaemia (ALL).Methods We analysed 76 matched bone marrow (BM) aspirate and PB specimens independently for the presence of ALL MRD by six-colour flow cytometry (FC).Results The overall rate of BM MRD-positivity was 24% (18/76) and PB was also MRD-positive in 22% (4/18) of BM-positive cases. We identified two cases with evidence of leukaemic cells in PB at the time of the extramedullary relapse that were interpreted as MRD-negative in BM.Conclusions The use of PB MRD as a non-invasive method for monitoring of systemic relapse may have added clinical and diagnostic value in patients with high risk of extramedullary disease.