@article {Alayed906, author = {Khaled Alayed and Karen Schweitzer and Amad Awadallah and Shashirekha Shetty and Samir Turakhia and Howard Meyerson}, title = {A multicolour flow cytometric assay for c-MYC protein in B-cell lymphoma}, volume = {71}, number = {10}, pages = {906--915}, year = {2018}, doi = {10.1136/jclinpath-2018-205075}, publisher = {BMJ Publishing Group}, abstract = {Aim Develop an objective assay to detect c-MYC protein expression using multiparametric flow cytometry (FCM) as an alternative to immunohistochemistry (IHC).Methods 57 patient samples and 11 cell line samples were evaluated. Cell suspensions were obtained and c-MYC staining was performed in combination with CD45 and CD19 and, in some samples, CD10. The percentage of c-MYC+ cells by FCM was correlated with the percentage determined by IHC. The relationship between c-MYC protein expression and the presence of a c-MYC gene rearrangement in aggressive and high-grade lymphomas was also assessed.Results c-MYC expression by FCM and IHC demonstrated a high degree of correlation in a training set of 33 patient cases, r=0.92, 11 cell line samples, r=0.81 and in a validation set of 24 aggressive and high-grade B-cell lymphomas, r=0.85. c-MYC gene was rearranged by fluorescence in situ hybridisation in 6/9 samples with high c-MYC expression (\>40\%) by FCM and 6/14 by IHC.Conclusions We have developed a reliable multicolour FCM assay to detect c-MYC expression suitable for clinical laboratories that should be helpful to accurately quantify c-MYC expression in B-cell lymphomas.}, issn = {0021-9746}, URL = {https://jcp.bmj.com/content/71/10/906}, eprint = {https://jcp.bmj.com/content/71/10/906.full.pdf}, journal = {Journal of Clinical Pathology} }