PT  - JOURNAL ARTICLE
AU  - Mara Ridolfi
AU  - Michele Paudice
AU  - Sandra Salvi
AU  - Luca Valle
AU  - Marina Gualco
AU  - Antonio Perasole
AU  - Luca Anselmi
AU  - Roberto Fiocca
AU  - Luca Mastracci
AU  - Federica Grillo
TI  - Agar pre-embedding of small skin biopsies: real-life benefits and challenges in high throughput pathology laboratories
AID  - 10.1136/jclinpath-2018-205680
DP  - 2019 Jun 01
TA  - Journal of Clinical Pathology
PG  - 448--451
VI  - 72
IP  - 6
4099  - http://jcp.bmj.com/content/72/6/448.short
4100  - http://jcp.bmj.com/content/72/6/448.full
SO  - J Clin Pathol2019 Jun 01; 72
AB  - Paraffin embedding of small, thin tissue samples requires specific expertise for optimal orientation before tissue sectioning. This study evaluates the real-life utility of the agar pre-embedding technique for small skin biopsies with regards to lengthening of work times, problems in orientation (re-embedding) and ancillary techniques (immunohistochemistry and in situ hybridisation) between two high work flow pathology laboratories, one of which routinely uses the agar pre-embedding technique and one which does not. The mean time required for pre-embedding in agar was 30.4 s, but time for paraffin embedding for agar pre-embedded samples was shorter than the traditional method (177 vs 296 s; p<0.005). The number of skin samples requiring re-embedding was significantly higher with the traditional embedding method (p<0.005). No problems in immunoreactivity were observed in all 1900 reactions performed with 17 different antibodies. Fluorescence in situ hybridisation analysis was optimised with a prolonged protease K incubation time (21 vs 18 min).