TY - JOUR T1 - Prognostic impact of p16 and PD-L1 expression in patients with oropharyngeal squamous cell carcinoma receiving a definitive treatment JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 542 LP - 549 DO - 10.1136/jclinpath-2019-205818 VL - 72 IS - 8 AU - Fumihiko Sato AU - Takeharu Ono AU - Akihiko Kawahara AU - Toshihiko Kawaguchi AU - Hisaichiro Tanaka AU - Kazuhide Shimamatsu AU - Tatsuyuki Kakuma AU - Jun Akiba AU - Hirohito Umeno AU - Hirohisa Yano Y1 - 2019/08/01 UR - http://jcp.bmj.com/content/72/8/542.abstract N2 - Aims Limited information is available regarding the precise differences in the tumour immune microenvironment (TIM) of patients with human papilloma virus (HPV)-associated and non-HPV-associated oropharyngeal squamous cell carcinoma (OPSCC). Here, we retrospectively reviewed 137 patients with OPSCC treated with a definitive treatment to identify molecular relationships in the TIM.Materials and methods We used immunohistochemical analysis to assess p16 status, programmed death ligand 1 (PD-L1) level, and/or CD8+ tumour-infiltrating lymphocyte (TIL) density, followed by prognostic evaluation of these immune-related parameters.Results Multivariate analyses demonstrated that PD-L1 level on immune cells but not on tumour cells or CD8+ TIL density was a significant predictive factor of disease-free survival (DFS) and overall survival (OS). Additionally, subgroup analyses demonstrated that patients positive for p16 and PD-L1 expression on immune cells had favourable DFS and OS, whereas patients negative for p16 and PD-L1 expression on immune cells showed worse DFS and OS.Conclusions We demonstrated that PD-L1 expression on immune cells but not tumour cells might represent a useful prognostic biomarker in patients with OPSCC receiving a definitive treatment. We propose that a co-assessment of p16 and PD-L1 expression on immune cells would have greater prognostic potential compared with evaluation of each factor alone in patients with OPSCC. ER -