PT  - JOURNAL ARTICLE
AU  - Melek Ahmed
AU  - Glenn Broeckx
AU  - Geert Baggerman
AU  - Karin Schildermans
AU  - Patrick Pauwels
AU  - Amaryllis H Van Craenenbroeck
AU  - Amelie Dendooven
TI  - Next-generation protein analysis in the pathology department
AID  - 10.1136/jclinpath-2019-205864
DP  - 2020 Jan 01
TA  - Journal of Clinical Pathology
PG  - 1--6
VI  - 73
IP  - 1
4099  - http://jcp.bmj.com/content/73/1/1.short
4100  - http://jcp.bmj.com/content/73/1/1.full
SO  - J Clin Pathol2020 Jan 01; 73
AB  - Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications.