RT Journal Article
SR Electronic
T1 An introduction of an easy-operating and economical technique for tissue microarray preparation
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 403
OP 407
DO 10.1136/jclinpath-2019-206142
VO 73
IS 7
A1 Yi-Jing Chen
A1 Chun-Mei Yang
A1 Jiang-Sheng Huang
A1 Ping Wang
A1 Yan-Hua Lv
A1 Cheng Tang
A1 Wei Deng
YR 2020
UL http://jcp.bmj.com/content/73/7/403.abstract
AB Aim Tissue microarray (TMA) is a powerful and effective tool for in situ tissue analysis. However, manual TMA construction methods showed varied qualities. This study aimed to raise a standardised TMA preparation technique that can be easily operated and is economical.Methods A sampling needle was used to punch the tissue rods from the donor block and holes in the recipient block. To indicate the dots’ positions and ensure vertical punching, a novel auxiliary device made using commercial three-dimensional printing technology was attached. The TMA block was made up of tissue rods and a recipient block.Results A 77-rod (7×11) TMA block was constructed. The rows and columns were fixed in straight lines. There was no specimen loss during the process of embedding.Conclusions An alternative method for the construction of TMA blocks that met the basic requirement of many laboratories and can be effortlessly performed was presented.