TY - JOUR T1 - Comparison between two different next generation sequencing platforms for clinical relevant gene mutation test in solid tumours JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 602 LP - 604 DO - 10.1136/jclinpath-2019-206422 VL - 73 IS - 9 AU - Silvia Bessi AU - Francesco Pepe AU - Marco Ottaviantonio AU - Pasquale Pisapia AU - Umberto Malapelle AU - Giancarlo Troncone AU - Mauro Biancalani Y1 - 2020/09/01 UR - http://jcp.bmj.com/content/73/9/602.abstract N2 - In the present study, we analysed 44 formalin fixed paraffin embedded (FFPE) from different solid tumours by adopting two different next generation sequencing platforms: GeneReader (QIAGEN, Hilden, Germany) and Ion Torrent (Thermo Fisher Scientific, Waltham, Massachusetts, USA). We highlighted a 100% concordance between the platforms. In addition, focusing on variant detection, we evaluated a very good agreement between the two tests (Cohen’s kappa=0.84) and, when taking into account variant allele fraction value for each variant, a very high concordance was obtained (Pearson’s r=0.94). Our results underlined the high performance rate of GeneReader on FFPE samples and its suitability in routine molecular predictive practice. ER -