TY - JOUR T1 - Detection of COX-2 in liquid biopsy in patients with breast cancer JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 826 LP - 829 DO - 10.1136/jclinpath-2020-206576 VL - 73 IS - 12 AU - Camilla Paulla de Souza AU - Beatriz Alves AU - Jaques Waisberg AU - Fernando Fonseca AU - Alipio de Oliveira Carmo AU - Flavia Gehrke Y1 - 2020/12/01 UR - http://jcp.bmj.com/content/73/12/826.abstract N2 - Aims To determine the expression of the cyclooxygenase-2 (COX-2) gene in patients with breast cancer attended at the Centro Universitário Saúde ABC/Faculdade de Medicina do ABC (CUS-ABC/FMABC) outpatient clinic. Breast cancer is the most common cancer in women worldwide. More than two million new cases are reported annually. An overexpression of COX-2 has been observed in many cancers. COX-2 is related to parameters of cancer aggressiveness, including tumour size, positive nodal state and lower survival, and to angiogenesis and resistance to apoptosis.Methods 15 mL of peripheral blood was obtained from 34 patients and 21 healthy women. The extracellular RNA of QIAamp RNA was submitted to an RNA sequestration kit for RNA reverse transcriptase. Quantitative real-time PCR was performed using COX-2-specific oligonucleotides and the endogenous Glyceraldehyde-3-Phosphate Dehydrogenase gene.Results The mean remission time was 53 years. The mean progression time was 33 months. The difference observed between the patient and control groups in median COX-2 expression (p<0.001) was significant.Conclusions Patients with breast cancer showed a higher mean COX-2 expression in peripheral blood samples at diagnosis than the control group. Since this information could prove important in the diagnosis and prognosis of breast cancer, further research is required on larger patient samples. ER -