TY - JOUR T1 - Severe hypercalcaemia in an infant JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 355 LP - 357 DO - 10.1136/jclinpath-2020-206697 VL - 74 IS - 6 AU - María Julia Martín AU - Kelly Maury Fuentes AU - María Belén Novoa Díaz AU - Gabriel Cao AU - Graciela Gigola AU - Oscar Brunetto AU - Claudia Gentili Y1 - 2021/06/01 UR - http://jcp.bmj.com/content/74/6/355.abstract N2 - A 3-month-old male patient arrived at Children’s Hospital ‘Dr. Pedro de Elizalde’ (Buenos Aires, Argentina) with irritability, rejection of food and altered neurobehavioral development. An abnormal mass was found in the right flank on palpation of the abdomen.Laboratory tests showed high concentrations of total serum calcium: 6.24 mmol/L (range of normal values in this age group: 2.20–2.82 mmol/L); low concentrations of parathormone (PTH): 0.175 pmol/L (normal 1.06–7.42 pmol/L) and phosphorus: 0.935 mmol/L (normal: 1.55–2.39 mmoL). Vitamin D measurements of 25(OH) showed normal values (63 nmol/L), considering the age group, where the vitamin D insufficiency is defined in the range of concentrations between 30 nmol/L and 50 nmol/L. Other laboratory test values, including blood cell count, were within normal limits.He was hydrated and initially treated with pamidronate and calcitonin but hypercalcaemia did not revert.Imaging-based proceduresAbdominal ultrasound and computed axial tomography images (figure 1A) revealed a retroperitoneal heterogeneous complex mass located between the liver and the right kidney, with variable pattern and calcifications, measuring 0.1×0.06×0.06 m. Both kidneys and adrenal glands preserved normal anatomical structure, the spleen was homogeneous. This type of lesion was not observed in other organs or tissues and no free liquid was found. Diagnostic angiography evidenced a highly vascularised tumour, irrigated by the middle colic artery (figure 1B).Figure 1 Clinical procedures for the diagnosis and treatment of the patient (A–C). (A) Computed axial tomography, (B) angiography and (C) tumour mass removed by surgical resection. Analysis of the tumour tissue biopsy (D–I). (D) Typical morphology of neoplastic cells coexisting with peripheral inflammatory infiltrates and (E) intracytoplasmic vacuoles representing small vascular lumina (black arrows) (H&E and PAS, 400×). (F) Reticulin expression (Gordon & Sweets reticulin stain, 400×). Expression of (G) vimentin and (H) CD31 (immunohistochemistry, 400×). (I) Immunostaining for Ki 67 (immunohistochemistry, 400×).Histology revealed spindle to polygonal-shaped neoplastic cells with abundant eosinophilic cytoplasm, arranged in … ER -